Leonardo Stari

Leonardo Stari

Specially Appointed Research Fellow at Tohoku University

Mei Lab · Selenium Bioremediation · Digital Biosphere (MEXT)

About Me

Leonardo Stari

Hi, I am Leonardo (Leo) Stari

I am currently a Specially Appointed Research Fellow at Tohoku University, conducting research in the Mei Lab (Graduate School of Environmental Studies). My work bridges experimental microbiology and computational modeling, focusing on the bioremediation of water and air pollutants, as well as predictive microbial ecology. By combining wet-lab isolation of novel degraders with next-generation AI architectures, I aim to transform how we understand and utilize microbial communities for environmental restoration.

Originally from Santiago, Chile, I moved to Sendai, Japan, in 2016. I hold a PhD in Environmental Chemistry and possess a diverse professional background that spans from IT project engineering to high-performance computing and wet-lab research.

Outside of the lab, I enjoy walking, swimming, and reading novels and manga. I am also an avid gamer, enjoying titles like World of Warcraft and Pokémon.

Career Timeline

2026 – Present
Specially Appointed Research Fellow (特任研究員)
Tohoku University, Mei Lab (環境科学研究科) · Visit Lab Website →
April 2022 – March 2026
Assistant Professor (Research)
Tohoku University, Digital Biosphere Project
2018 – Mar 2022
Ph.D., Environmental Studies
Tohoku University
2016 – 2018
Master's degree, Environmental Engineering Technology / Environmental Technology
Tohoku University
Bioremediation of chlorinated methanes
2013 – 2016
IT Project Engineer
Novakem, Santiago, Chile
2006 – 2012
Engineer's degree, Biotechnology
Universidad de Chile
Seishin, Renewable Energies Lab. (2006-2009) | Intro. Japanese course & assistant teacher (2007-2009) | Summer school teacher | Thesis in biofuels
2004 – 2005
Bachelor, Science
Universidad de Chile
Bachelor degree, thesis in bioethics
ORCID ResearchGate LinkedIn

Skills & Languages

🌐 Languages

  • 🇪🇸 Spanish (Native)
  • 🇬🇧 English (Advanced)
  • 🇯🇵 Japanese (Advanced)
  • 🇫🇷 French (Intermediate)

🧪 Wet Lab Techniques

  • Microbial Isolation & Enrichment Cultures
  • Bioreactor Design & Scale-up
  • ICP-MS Quantitative Analysis
  • Plant Physiology & Field Work

💻 Dry Lab & Computational Techniques

  • Deep Learning & AI (LSTM, JEPA, xLSTM)
  • Metagenomics & Bioinformatics Pipelines (HPC)
  • Illumina & Nanopore Sequencing Analysis
  • Python, MATLAB & Java Scripting

Research Interests

My academic path is driven by a goal to elucidate and harness microbial processes for environmental benefit. My work combines wet-lab experimentation with data-driven modeling.

Bioremediation of Water & Air Pollutants

I focus on harnessing microbial processes to degrade persistent organic pollutants and heavy metals from contaminated water and air. My work spans the isolation of novel degraders, functional genomics, and bioreactor-scale bioaugmentation strategies. Key examples include the isolation of Pseudomonas sp. Stari2 for aerobic carbon tetrachloride degradation, and current efforts targeting selenate-reducing consortia for mine wastewater treatment.

See: Stari et al. (PhD Thesis, 2022) on CT-degrading consortia, and ongoing selenium bioremediation projects at Mei Lab.

Microbial Ecology & Predictive AI

To bridge the gap between isolate characterization and ecosystem function, I study how microbial communities assemble and respond to environmental shifts. Leveraging functional shotgun metagenomics and high-resolution time-series data, I apply next-generation AI architectures (LSTM, JEPA, xLSTM) to transform microbial ecology into a predictive science.

See: Stari et al. (2026), ISME Communications — "Carbon Source Acts as a Deterministic Filter Shaping Microbial Succession..."

Latest Publication

📢 NEW

Carbon Source Acts as a Deterministic Filter Shaping Microbial Succession and Rare-Abundant Decoupling in Soil Bacterial Communities

View Paper →

ISME Communications | Published: April 20, 2026 | DOI: 10.1093/ismeco/ycag108

We investigated how chemically diverse carbon sources act as ecological filters shaping soil bacterial communities. Null model analysis confirmed the carbon source as the primary deterministic filter, enforcing high reproducibility (homogeneous selection governing ~74% of assembly among replicates) and overriding stochastic effects. Crucially, abundant (>1%) and rare (<0.1%) taxa exhibited decoupled assembly mechanisms — while abundant taxa were driven by dispersal limitation (~59%) and variable selection, the rare biosphere displayed a temporal regime shift, transitioning from stochastic isolation to strong deterministic selection (~50%) during later successional stages. This reframes the rare biosphere as a "latent responder" reservoir recruited by metabolic byproducts rather than the primary substrate.

Research Metrics

Citations
26
h-index
3
Publications
8

Metrics based on Scopus / Google Scholar data. Updated via Python script.

Current Projects

🧪 Biological Selenium Removal from Mine Wastewater

Targeting the underexplored selenate (Se⁶⁺) reduction pathway, this project isolates and characterizes selenate-reducing bacterial consortia from selenium-affected mine wastewater. The approach combines strain isolation with bioaugmentation strategies, aiming to achieve selenium concentrations below 10 ppb in treated effluent through lab-scale and pilot reactor testing.

🤖 AI-Driven Prediction of Bacterial Communities

Leveraging functional shotgun metagenomic data and high-resolution time-series sequencing, this project applies next-generation AI architectures (LSTM, JEPA, xLSTM) to predict microbial community dynamics and succession patterns. The goal is to transform microbial ecology from an observation-heavy field into a highly predictive science.

💨 Bioremediation of Contaminated Air

Investigating microbial degradation of volatile organic compounds (VOCs) and persistent gaseous pollutants. Building on previous work with carbon tetrachloride, this project explores biofilter and bioreactor systems for treating industrial and environmental air emissions.

Publications

Selected Talks & Presentations

🎤 Conference Oral Presentations

  • Deep Neural Networks for Predicting OTU Dynamics in Soil Bacterial Communities
    Stari, L.; Kishida, K.; Ohtsubo, Y.; Nagata, Y. · JSBBA 2025 日本農芸化学会 · 📍 Sapporo, Japan · Mar 2025
  • Deterministic Shaping of Soil Bacterial Communities: Interplay of Rare Species and Environmental Selection
    Stari, L.; Kato, H.; Ohtsubo, Y.; Nagata, Y. · JSBBA 2024 日本農芸化学会 · 📍 Tokyo, Japan · Mar 2024
  • The Succession of Taxonomic Structure and Metagenome Composition of Bacterial Community Cultured with Different Carbon Sources
    Stari, L.; Kato, H.; Ohtsubo, Y.; Nagata, Y. · 日本農芸化学会 2023 · 📍 Hiroshima, Japan · Mar 2023
  • Assessment of a Carbon Tetrachloride Degrading Consortia under Anaerobic Conditions
    Stari, L.; Inoue, C.; Chien, M. · 第13回 細菌学若手コロッセウム in みやぎ蔵王 · 📍 Miyagi, Japan · Aug 2019

🖼️ Conference Poster Presentations

  • Assessment of Carbon Tetrachloride Degrading Consortia under Anaerobic Conditions
    Stari, L.; Inoue, C.; Chien, M. · ASM Microbe 2019 · 📍 California, USA · Jun 2019

Contact

Feel free to reach out for collaborations or inquiries!

lstari@tohoku.ac.jp

Alternative Email

📍 〒980-8579 宮城県仙台市青葉区荒巻字青葉6-6-20
東北大学大学院 環境科学研究科研究棟 4F (Mei Lab)